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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Regulation of Expression at Multiple Steps

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Related Experiment Video

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

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Published on: April 25, 2022

MicroRNA regulation and tissue-specific protein interaction network.

Wenliang Zhu¹, Lei Yang, Zhimin Du

¹Institute of Clinical Pharmacology, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.

|October 8, 2011

Summary

MicroRNAs (miRNAs) fine-tune protein levels, impacting human biology. miRNAs targeting common genes often regulate tissue-specific genes, suggesting crucial roles in diverse tissues.

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Area of Science:

Molecular Biology
Genomics
Systems Biology

Background:

MicroRNAs (miRNAs) are key regulators of protein abundance, influencing numerous biological processes.
The functional impact of a single miRNA is constrained by tissue-specific gene expression, as only a subset of its targets is active.

Purpose of the Study:

To investigate how tissue-specific gene expression influences miRNA regulation of target genes.
To analyze the characteristics of miRNA regulation in different human tissues.

Main Methods:

Construction of tissue-specific protein interaction networks for ten major human tissue types.
Analysis of miRNA regulation patterns in relation to tissue-specific gene expression.

Main Results:

Commonly expressed proteins are subject to more intensive, lower-cost miRNA control compared to tissue-specific proteins.
MiRNAs targeting broadly expressed genes tend to regulate more tissue-specific genes.
MiRNA regulatory strategies vary across tissues, indicating diverse tissue-specific regulation modes.

Conclusions:

MiRNAs that interact with a higher proportion of commonly expressed genes are likely to play significant tissue-specific roles.
Understanding miRNA-gene interactions within tissue-specific networks is crucial for deciphering their biological functions.