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Related Experiment Video

Updated: May 28, 2026

Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

Bioenergetic profiling of zebrafish embryonic development.

Krista D Stackley1, Craig C Beeson, Jennifer J Rahn

  • 1Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, South Carolina, United States of America.

Plos One
|October 8, 2011
PubMed
Summary

We developed a new method to screen bioenergetics and mitochondrial function in zebrafish embryos. This assay allows sensitive detection of drug and environmental effects on early development.

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Area of Science:

  • Developmental Biology
  • Mitochondrial Biology
  • Toxicology

Background:

  • Debilitating conditions are often linked to bioenergetic defects, but screening for genetic or chemical causes is challenging.
  • A physiologically relevant assay for whole-organism bioenergetics is needed, particularly for early life stages where developmental perturbations can impact aging-related disease susceptibility.
  • Existing methods lack the ability to screen bioenergetics in whole, developing organisms.

Purpose of the Study:

  • To establish a multiplexed assay for screening bioenergetics and mitochondrial function in developing zebrafish embryos.
  • To provide a high-throughput, sensitive, and reproducible method for assessing the impact of drugs and environmental agents on embryonic bioenergetics.
  • To investigate the correlation between bioenergetic function and developmental stage in zebrafish.

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Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research
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Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research

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Related Experiment Videos

Last Updated: May 28, 2026

Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

High Throughput Danio Rerio Energy Expenditure Assay
08:35

High Throughput Danio Rerio Energy Expenditure Assay

Published on: January 27, 2016

Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research
04:11

Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research

Published on: October 10, 2025

Main Methods:

  • Developed a multiplexed assay to measure respiration and acid extrusion in zebrafish embryos from 3 to 48 hours post-fertilization.
  • Utilized specific pharmacological inhibitors to quantify respiratory coupling to various bioenergetic pathways, including ATP turnover and proton leak.
  • Employed a multiwell plate format for high-throughput screening capabilities.

Main Results:

  • Demonstrated the ability to assay bioenergetics and mitochondrial function in developing zebrafish embryos.
  • Quantified respiratory coupling and identified correlations between ATP turnover, proton leak, and developmental stage.
  • Validated the assay's sensitivity and reproducibility in proof-of-principle experiments.

Conclusions:

  • The developed zebrafish embryo assay is a powerful tool for screening bioenergetic and mitochondrial function.
  • This method enables the assessment of drug and environmental agent effects on early life bioenergetics with high throughput.
  • The assay provides a physiologically relevant model for understanding links between bioenergetic defects and disease susceptibility.