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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Alternative splicing adds a new loop to the circadian clock.

Ezequiel Petrillo1, Sabrina E Sanchez2, Alberto R Kornblihtt1

  • 1IFIBYNE; FCEyN; UBA-CONICET; C1428EGA; Buenos Aires, Argentina.

Communicative & Integrative Biology
|October 8, 2011
PubMed
Summary

Protein arginine methyl transferase 5 (PRMT5) regulates alternative splicing in plant and animal circadian clocks. This mechanism fine-tunes biological timing by integrating alternative splicing into the core circadian program.

Keywords:
Arabidopsis thalianaDrosophila melanogasterPRMT5alternative splicingarginine methyltransferasecircadian clockepigenetictranscriptional regulation

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Area of Science:

  • Molecular Biology
  • Chronobiology
  • Plant Science

Background:

  • Circadian clocks regulate physiological processes through molecular feedback loops.
  • These loops involve transcriptional, translational, and post-translational modifications.
  • Alternative splicing's role in circadian timing remains largely unexplored.

Purpose of the Study:

  • To investigate the role of protein arginine methyl transferase 5 (PRMT5) in the plant circadian clock.
  • To explore PRMT5's involvement in alternative splicing within the circadian network.
  • To determine the significance of alternative splicing in biological clock function.

Main Methods:

  • Investigated PRMT5 function in Arabidopsis thaliana circadian clock.
  • Examined PRMT5's regulation of alternative splicing in key clock mRNAs.
  • Assessed PRMT5's role in the circadian network of Drosophila melanogaster.

Main Results:

  • Provided evidence that PRMT5 is part of a novel loop in the Arabidopsis circadian clock.
  • Demonstrated PRMT5 regulates alternative splicing of clock mRNAs.
  • Found PRMT5 influences alternative splicing and circadian networks in Drosophila, potentially at the output level.

Conclusions:

  • Alternative precursor messenger RNA (pre-mRNA) splicing is an integral part of the circadian program.
  • Alternative splicing may be a key mechanism for fine-tuning biological clocks.
  • PRMT5's role highlights the fundamental relevance of splicing in circadian regulation across species.