Related Experiment Video
Updated: May 28, 2026
Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
Cell quality of apheresis-derived platelets treated with riboflavin-ultraviolet light after resuspension in platelet
Susanne M Picker1, Marie E Tauszig, Birgit S Gathof
1Transfusion Medicine, University Hospital of Cologne, Cologne, Germany.
Background:
Previously, we evaluated the Mirasol pathogen reduction technology (PRT) system on platelet (PLT) function before resuspension. We now evaluated this system in the presence of PLT additive solution (PAS).
Study Design And Methods:
Double-dose PLTs (n = 15) were generated using a commercially available apheresis system (Trima, Version 5.2, CaridianBCT) allowing for the resuspension in SSP+ (MacoPharma) immediately after collection. Paired units (n = 30) were PRT treated (M) or remained untreated (C) and analyzed for metabolism (pH, pO(2) , glucose, lactate, adenosine triphosphate [ATP]), swirl, hypotonic shock response (HSR), turbidometric aggregation, CD62P expression, annexin A5 and lactate dehydrogenase (LDH) release, mitochondrial enzymatic reduction activity (MTS), transmembrane mitochondrial potential (Δψ), and surface coverage (SC) during shear-induced adhesion throughout 8 days of storage.
Results:
As seen previously, PRT treatment of PLT units, containing a mean of 3.9 × 10(11) ± 0.3 × 10(11) PLTs in 397 ± 10 mL with a 32% to 34% plasma carryover, was associated with significantly (p < 0.001) increased cell activation, acidity, and glycolytic flux. PRT treatment appeared to up regulate both oxidative pathway and adhesional properties as evidenced by significantly higher MTS reduction, oxygen consumption, and shear-induced SC on Day 1 (p ≤ 0.016). While no significant differences were found for LDH release and ATP content (except for Day 8), M units were significantly inferior (p ≤ 0.021) for aggregation (TRAP-6); for Δψ and annexin A5 release (by Day 5); and for swirl, HSR, and MTS reduction (by Day 7).
Conclusion:
PRT treatment in the presence of PAS was comparable to PRT treatment before resuspension preserving ATP content and mitochondrial function.
Insights
Mirasol pathogen reduction technology (PRT) with platelet additive solution (PAS) maintained platelet function and mitochondrial health during storage. This method is comparable to PRT used before resuspension, ensuring platelet viability.
Area of Science:
- Blood banking and transfusion medicine
- Platelet biology and function
- Pathogen reduction technologies
Background:
- Previous studies evaluated Mirasol PRT on platelet function before resuspension.
- This study assessed Mirasol PRT in the presence of platelet additive solution (PAS).
Purpose of the Study:
- To evaluate the impact of Mirasol PRT in PAS on platelet function and metabolism during storage.
- To compare PRT with PAS to PRT before resuspension.
Main Methods:
- Platelets (PLTs) were collected and resuspended in SSP+ (PAS).
- Paired units were treated with Mirasol PRT (M) or left untreated (C).
- Analyzed various parameters including metabolism, activation markers, aggregation, and mitochondrial function over 8 days.
Main Results:
- PRT treatment increased cell activation, acidity, and glycolytic flux.
- PRT enhanced oxidative pathways and adhesion properties initially.
- However, PRT-treated units showed impaired aggregation, mitochondrial potential, and hypotonic shock response later in storage.
Conclusions:
- Mirasol PRT in PAS preserves ATP content and mitochondrial function, similar to PRT before resuspension.
- The study demonstrates the feasibility of using Mirasol PRT with PAS for platelet processing.
More Related Videos
05:49Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
Published on: November 29, 2024
12:40Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012