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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes
Niall F Davis1, Rory Mooney, Anna V Piterina
1Department of Urology, Mid-Western Regional Hospital, Limerick, Ireland. nialldavis2001@yahoo.com
Urology
|October 11, 2011
Summary
A novel urinary bladder bioreactor enhanced urothelial cell growth on extracellular matrix scaffolds. This tissue-engineering advancement shows promise for future urologic applications compared to static culture methods.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Urology
Background:
- Tissue engineering aims to regenerate or repair damaged tissues.
- Urinary bladder reconstruction faces challenges in achieving functional restoration.
- Bioreactors offer controlled environments for cell culture and tissue development.
Purpose of the Study:
- To design and build a urinary bladder bioreactor.
- To assess cell viability and proliferation on scaffolds within the bioreactor.
- To compare bioreactor culture with static conditions for urologic tissue engineering.
Main Methods:
- A bioreactor was engineered to simulate physiologic bladder pressures.
- Human urothelial cells were cultured on porcine extracellular matrix scaffolds.
- Cell viability and proliferation were quantified over 5 days using viability assays and fluorescent markers.
Main Results:
- No significant difference in cell viability at day 3.
- Significantly greater urothelial cell numbers in the bioreactor by day 4 (P = .009) and day 5 (P = .006).
- Scaffold integrity was confirmed via scanning electron microscopy and DAPI staining.
Conclusions:
- The bioreactor significantly enhanced urothelial cell growth on extracellular matrix scaffolds compared to static culture.
- This bioreactor prototype represents a step forward in urinary bladder tissue-engineering technology.
