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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: May 28, 2026

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Cell adhesion control on photoreactive phospholipid polymer surfaces.

Batzaya Byambaa¹, Tomohiro Konno, Kazuhiko Ishihara

¹Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Tokyo 113-8656, Japan.

Colloids and Surfaces. B, Biointerfaces

|October 11, 2011

Summary

Researchers developed a novel photoreactive polymer surface to easily detach cells from culture dishes using UV light. This method ensures cell viability for future studies and cell passage.

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Last Updated: May 28, 2026

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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

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Published on: January 29, 2022

Area of Science:

Biomaterials Science
Cell Biology
Surface Chemistry

Background:

Non-invasive cell recovery is crucial for cell passage and characterization.
Current methods for cell detachment can impact cell viability and function.
Controlling cell adhesion at the substrate interface is key for cell manipulation.

Purpose of the Study:

To develop a photoreactive polymer surface for controlled cell adhesion and detachment.
To investigate the properties of a novel phospholipid polymer (PMB-PL) for cell culture applications.
To demonstrate a UV-light-triggered method for non-invasive cell recovery.

Main Methods:

Synthesis of a photoreactive phospholipid polymer (PMB-PL) incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) and a photolabile nitrobenzyl group.
Coating cell culture substrates with the PMB-PL polymer to create a photoreactive surface.
Surface characterization using techniques to assess photoresponse and surface properties (e.g., ζ-potential, hydrophilicity).
Evaluating cell adhesion and detachment upon UV irradiation, assessing cell viability post-detachment.

Main Results:

The PMB-PL polymer exhibited a strong photoresponse upon UV irradiation.
The PMB-PL surface effectively supported cell adhesion prior to UV exposure.
UV irradiation converted the PMB-PL coating to a neutral, hydrophilic surface, inducing cell detachment.
Detached cells maintained high viability, indicating a non-invasive recovery process.

Conclusions:

The developed PMB-PL photoreactive surface enables controlled cell attachment and detachment.
UV-triggered cell detachment offers a non-invasive method for cell recovery, preserving cell viability.
This technology holds significant promise for cell function investigation and cell passage in biological research.