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Related Experiment Video

Updated: May 28, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Mitochondrial complex III ROS regulate adipocyte differentiation.

Kathryn V Tormos1, Elena Anso, Robert B Hamanaka

  • 1Division of Pulmonary and Critical Care, Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA.

Cell Metabolism
|October 11, 2011
PubMed
Summary
This summary is machine-generated.

Mitochondrial metabolism and reactive oxygen species (ROS) are crucial drivers of adipocyte differentiation, not mere byproducts. This study reveals ROS generation is essential for initiating fat cell development.

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Last Updated: May 28, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
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Published on: February 3, 2023

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Published on: May 19, 2023

Area of Science:

  • Cell Biology
  • Metabolic Research
  • Stem Cell Science

Background:

  • Adipocyte differentiation involves increased mitochondrial metabolism, but its essentiality remains unclear.
  • The role of mitochondrial activity and reactive oxygen species (ROS) in adipogenesis is debated.

Purpose of the Study:

  • To determine if increased mitochondrial metabolism and ROS are essential for adipocyte differentiation.
  • To investigate the signaling pathways and specific mitochondrial components involved in ROS generation during adipogenesis.

Main Methods:

  • Primary human mesenchymal stem cells were differentiated into adipocytes.
  • mTORC1 signaling, mitochondrial biogenesis, and ROS generation were assessed.
  • Mitochondrial-targeted antioxidants and genetic manipulation of mitochondrial complex III were employed.

Main Results:

  • An early increase in mitochondrial metabolism, biogenesis, and ROS generation was observed during adipocyte differentiation, dependent on mTORC1.
  • Inhibition of mitochondrial ROS production blocked adipocyte differentiation, an effect reversed by exogenous hydrogen peroxide.
  • ROS specifically generated from mitochondrial complex III was found to be critical for initiating differentiation.

Conclusions:

  • Mitochondrial metabolism and ROS generation are causal factors, not byproducts, in promoting adipocyte differentiation.
  • ROS signaling originating from mitochondrial complex III is essential for initiating the adipogenic process.