Related Experiment Video
Updated: May 28, 2026
Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models
Published on: June 30, 2023
Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy
Harpreet Singh1, Rong Lu, Pedro Felipe Gardeazábal Rodríguez
1Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
None:
The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~28nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~33, ~55 and ~83nm.
