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Measurement of bacterial volume by transmission-through-dye imaging
Suzanne L Lababidi1, Mariana Pelts, Moumita Moitra
1Department of Biological Sciences, Kent State University, Kent, OH 44242, USA.
Abstract:
Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.
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