Live-cell 3D super-resolution imaging in thick biological samples

Related Experiment Video

Updated: May 28, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Francesca Cella Zanacchi1, Zeno Lavagnino, Michela Perrone Donnorso

  • 1Istituto Italiano di Tecnologia, Genova, Italia.

Nature Methods
|October 11, 2011
PubMed

More Related Videos

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.