Related Experiment Video
Updated: May 28, 2026

Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese
Herbert Seiler1, Anne Bleicher1, Hans-Jürgen Busse2
1Department of Microbiology (ZIEL), Technische Universität München, Weihenstephaner Berg 3, D-85350 Freising, Germany.
Abstract:
A novel halophilic, Gram-reaction-negative, strictly aerobic, non-motile, rod-shaped and oxidase- and catalase-positive bacterial strain, designated WCC 4520(T), was isolated from a semi-hard, Raclette-type cheese. The colonies were yellow-orange; flexirubin-type pigments were not found. The strain hydrolysed gelatin, hippurate, tyrosine and Tweens 20 and 80. Optimal growth was observed with 6-8% (w/v) NaCl, at pH 7-8 and at 27-30 °C. The genomic DNA G+C content was 33.6 mol%. In phylogenetic analysis based on 16S rRNA gene sequences, strain WCC 4520(T) appeared to be a member of the family Flavobacteriaceae and the closest phylogenetic neighbours were identified as Psychroflexus gondwanensis DSM 5423(T) (94.0% 16S rRNA gene sequence similarity) and Psychroflexus salinarum CCUG 56752(T) (94.0%). The predominant cellular fatty acids were iso-C(15:0), anteiso-C(15:0), iso-C(15:1) G and iso-C(17:0) 3-OH. The only detected quinone was MK-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Minor polar lipids and traces of polyamines were also detected. On the basis of the data presented, strain WCC 4520(T) represents a novel species of the genus Psychroflexus, for which the name Psychroflexus halocasei sp. nov. is proposed. The type strain is WCC 4520(T) ( = LMG 25857(T) = CCUG 59705(T)).
Related Concept Videos
Bacterial Phylum Spirochaetes
Bacterial Phylum Actinobacteria
Microbes in the Production of Fermented Foods
Microbes in Food Production
Deep Sea Microbial Ecology
Bacterial Phylum Firmicutes

