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Updated: May 28, 2026

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
Published on: August 26, 2010
ESR Microscopy for Biological and Biomedical Applications
C S Shin1, C R Dunnam, P P Borbat
1National Biomedical Center for Advanced ESR Technology, Cornell University, Ithaca, NY 14853, USA.
None:
We report on electron-spin resonance microscopy (ESRM) providing sub-micron resolution (~700nm) with a high spin concentration sample, i.e. lithium phthalocyanine (LiPc) crystal. For biomedical applications of our ESRM, we have imaged samples containing rat basophilic leukemia (RBL) cells as well as cancerous tissue samples with a resolution of several microns using a water soluble spin probe, Trityl_OX063_d24. Phantom samples with the nitroxide spin label, (15)N PDT, were also imaged to demonstrate that nitroxides, which are commonly used as spin labels, may also be used for ESRM applications. ESRM tissue imaging would therefore be valuable for diagnostic or therapeutic purposes. Also, ESRM can be used to study the motility or the metabolism of cells in various environments. With further modification and/or improvement of imaging probe and spectrometer instrumentation sub-micron biological images should be obtainable, thereby providing a useful tool for various biomedical applications.
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