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Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

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Structural messenger RNA contains cytokeratin polymerization and depolymerization signals.

Malgorzata Kloc1, Paul Dallaire, Arkadiy Reunov

  • 1Department of Surgery, The Methodist Hospital and The Methodist Hospital Research Institute, 6565 Fannin Street, Houston, TX 77030, USA. mkloc@tmhs.org

Cell and Tissue Research
|October 12, 2011
PubMed
Summary
This summary is machine-generated.

VegT mRNA provides structural support for the Xenopus oocyte cytoskeleton. A specific RNA fragment containing a conserved hairpin structure induces and maintains cytokeratin filament depolymerization.

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • VegT mRNA has a previously identified structural, translation-independent role in Xenopus oocyte cytokeratin organization.
  • VegT mRNA depletion leads to cytokeratin network fragmentation in Xenopus oocytes, which can be rescued by synthetic VegT RNA injection.

Purpose of the Study:

  • To investigate the specific signals within VegT mRNA responsible for regulating cytokeratin filament dynamics.
  • To determine if a defined RNA fragment can replicate the structural functions of the full VegT mRNA.

Main Methods:

  • Injection of synthetic VegT RNA and a specific 300-nucleotide VegT RNA fragment into Xenopus oocytes.
  • Observation of cytokeratin filament organization and depolymerization/polymerization status.
  • Computational analysis of homologous Xenopus VegT mRNAs to identify conserved RNA structures.

Main Results:

  • The structural function of VegT mRNA relies on signals that induce, facilitate, and maintain cytokeratin filament depolymerization/polymerization.
  • A 300-nucleotide fragment of VegT RNA, when isolated, can induce and maintain cytokeratin filament depolymerization in Xenopus oocytes.
  • Computational analysis revealed a conserved base-pairing (hairpin) configuration within this 300-nucleotide region, suggesting a role in RNA/protein interactions.

Conclusions:

  • VegT mRNA contains specific functional regions that control cytokeratin filament dynamics.
  • A conserved hairpin structure within a 300-nucleotide fragment of VegT RNA is crucial for its structural role in Xenopus oocytes.
  • This structural role is likely mediated through RNA/protein interactions.