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Updated: May 28, 2026
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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Stable protein expression in mammalian cells using baculoviruses.
Andreas Lackner1, Emanuel Kreidl, Barbara Peter-Vörösmarty
1Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2011
Summary
This study presents a recombinant baculovirus for stable protein expression in mammalian cells. The engineered virus facilitates transgene expression, demonstrated by generating cancer cell lines expressing green fluorescent protein.
Area of Science:
- Biotechnology
- Molecular Biology
- Virology
Background:
- Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) is a baculovirus commonly used for protein expression in insect cells.
- Baculoviruses naturally infect arthropods and cannot replicate in mammalian cells.
- Engineered AcMNPV can enter mammalian cells for transgene expression.
Purpose of the Study:
- To describe the construction and application of a recombinant baculovirus for stable protein expression in mammalian cells.
- To demonstrate the utility of this system for generating engineered mammalian cell lines.
Main Methods:
- Construction of a recombinant baculovirus with a bicistronic expression cassette.
- Transduction of mammalian cell lines (glioblastoma and hepatocellular carcinoma).
- Puromycin selection for stable protein expression.
Main Results:
- Successful generation of mammalian cell lines stably expressing proteins.
- Demonstrated expression of green fluorescent protein in engineered glioblastoma and hepatocellular carcinoma cells.
Conclusions:
- Recombinant baculoviruses with bicistronic cassettes are effective tools for stable transgene expression in mammalian cells.
- This system provides a novel approach for generating engineered mammalian cell lines for research and biotechnology.
