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Updated: May 28, 2026
Accessing the Subdural Space of the Rodent Spinal Cord for Treatment Delivery
Published on: August 8, 2025
Unintentional subdural placement of epidural catheters during attempted epidural anesthesia: an anatomic study of
Miguel Angel Reina1, Clive B Collier, Alberto Prats-Galino
1Department of Clinical Medical Sciences and Applied Molecular Medicine Institute, CEU San Pablo University School of Medicine, Madrid, Spain. miguelangel.rei@terra.es
Background:
Although infrequent, subdural block is a complication of epidural anesthesia with obvious implications. Knowledge of the spinal subdural compartment (dura-arachnoid interface) may help elucidate controversies arising from evidence that subdural catheter placement is feasible and may be difficult to identify clinically.
Methods:
Samples of arachnoid lamina obtained during in vivo lumbosacral surgery (n = 4) and from cadavers (n = 6) were obtained and prepared for transmission electron microscopy and scanning electron microscopy. Subdural spaces were artificially produced in suitable samples, and an epidural catheter was inserted between the arachnoid and dura to compare the dimensions of meninges in relation to epidural catheters.
Results:
Scanning electron microscopy of the dural sac showed areas of continuity between the arachnoid lamina and dura mater and other parts with both membranes separated by a subdural space. Transmission electron microscopy allowed the study of such border zones, where alternating cellular and collagen layers could be seen. A layer rich in collagen fibers and some fibroblasts separated arachnoid and neurothelial cells (dural border cells). Few specialized membrane junctions were found among cells adjacent to collagen fibers. Dura mater had an average thickness of 260 to 400 μm, with a dural lamina of approximately 4 to 6 μm. In areas where the arachnoid appeared separated from the dural lamina, its thickness measured 35 to 45 μm. Catheters with a diameter of 700 μm were successfully inserted inside the subdural space, between the dura mater and the arachnoid lamina.
Conclusions:
Dura mater and arachnoid layers act as a single unit but may be pulled apart by traction forces during cadaver processing of the dural sac or in vivo placement of catheters. This generates subdural spaces, either parallel or concentric, because of the minimal resistance offered by the tissue, which may be explained by its few specialized membrane junctions.
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