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Double Whole Mount in situ Hybridization of Early Chick Embryos
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ER71 directs mesodermal fate decisions during embryogenesis.

Tara L Rasmussen1, Junghun Kweon, Mackenzie A Diekmann

  • 1Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.

Development (Cambridge, England)
|October 13, 2011
PubMed
Summary
This summary is machine-generated.

ER71 is crucial for specifying hematopoietic and endothelial cell lineages. Its absence causes these cells to develop into cardiac lineages instead, impacting early embryogenesis.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • ER71 (also known as Ets2-related transcription factor 1) is essential for embryonic development.
  • Mutant embryos lacking functional ER71 do not survive and fail to develop hematopoietic and endothelial cell lineages.

Purpose of the Study:

  • To elucidate the precise role of ER71 in regulating cell fate decisions during early embryogenesis.
  • To investigate how ER71 influences the development of mesodermal lineages, specifically hematopoietic, endothelial, and cardiac cells.

Main Methods:

  • Generation of Er71-EYFP and Er71-Cre transgenic mouse models.
  • Fluorescence-activated cell sorting (FACS) and transcriptional profiling of EYFP-expressing cells.
  • Genetic fate-mapping studies using the Er71-Cre model.
  • Analysis of an inducible embryonic stem/embryoid body system with ER71 manipulation.

Main Results:

  • In Er71-deficient embryos, there was an increase in EYFP-expressing cells, heightened cardiac gene expression, and reduced hemato-endothelial gene expression compared to wild-type controls.
  • Fate-mapping revealed that ER71-expressing cells normally differentiate into hematopoietic and endothelial lineages.
  • Loss of ER71 function redirected these precursor cells towards alternative mesodermal fates, notably cardiac lineages.
  • ER71 overexpression in embryonic stem cell models inhibited cardiogenesis.

Conclusions:

  • ER71 acts as a critical regulator of mesodermal fate determination.
  • ER71 specifies hematopoietic and endothelial lineages by actively suppressing alternative fates, such as the cardiac lineage.
  • These findings provide key insights into the molecular mechanisms governing early embryonic cell lineage specification.