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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: May 28, 2026

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
09:36

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

Efficient nanoparticle mediated sustained RNA interference in human primary endothelial cells.

Anindita Mukerjee1, Jwalitha Shankardas, Amalendu P Ranjan

  • 1Department of Molecular Biology & Immunology, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.

Nanotechnology
|October 13, 2011
PubMed
Summary
This summary is machine-generated.

Poly(D,L-lactide-co-glycolide) nanoparticles efficiently deliver short hairpin RNA (shRNA) for gene silencing in endothelial and cancer cells, showing high transfection rates and low toxicity compared to Lipofectamine.

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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

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Last Updated: May 28, 2026

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
09:36

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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Nanotechnology

Background:

  • Endothelial cells are crucial for vascular functions and disease pathogenesis, making them key targets for gene therapy.
  • RNA-mediated gene silencing offers therapeutic potential but faces challenges in endothelial cell transfection efficiency and toxicity.
  • Annexin A2 is implicated in various pathophysiological conditions, including angiogenesis and cancer.

Purpose of the Study:

  • To develop and evaluate poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles for efficient and safe delivery of short hairpin RNA (shRNA) targeting Annexin A2 in endothelial and cancer cells.
  • To compare the transfection efficiency and toxicity of PLGA nanoparticles with conventional Lipofectamine-mediated delivery.

Main Methods:

  • Formulation of shRNA Annexin A2 loaded PLGA nanoparticles with 57.65% plasmid encapsulation.
  • Transfection of primary retinal microvascular endothelial cells and human cancer cell lines.
  • Comparison of nanoparticle-based transfection with Lipofectamine-mediated transfection, assessing cell viability and Annexin A2 downregulation.

Main Results:

  • PLGA nanoparticles achieved high transfection efficiency (~97%) and sustained gene silencing compared to Lipofectamine.
  • Nanoparticle-based transfection exhibited minimal toxicity, with ~95% cell viability 24 hours post-transfection, contrasting with Lipofectamine's ~30% viability.
  • Effective downregulation of Annexin A2 was observed following nanoparticle-mediated shRNA delivery.

Conclusions:

  • PLGA nanoparticles provide an efficient and low-toxicity platform for siRNA delivery to human primary endothelial and cancer cells.
  • This nanoparticle-based approach holds promise as an adjuvant therapy for vascular diseases like diabetic retinopathy and age-related macular degeneration, as well as various cancers.