Related Experiment Video
Updated: May 28, 2026
06:06
Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
Published on: May 9, 2022
Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model
Masato Nagayoshi1, Tatsuji Nishihara, Keisuke Nakashima
1Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan.
ISRN Dentistry
|October 13, 2011
Summary
Diode laser combined with a photosensitizer effectively reduced endodontic pathogens in periapical lesions. This photodynamic therapy approach shows promise for treating apical periodontitis.
Area of Science:
- Endodontics
- Photodynamic Therapy
- Microbiology
Background:
- Photodynamic therapy (PDT) is increasingly used in endodontic treatments.
- Periapical lesions are often caused by endodontic pathogens.
- Investigating novel antimicrobial strategies for endodontic infections is crucial.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of diode laser irradiation combined with a photosensitizer against endodontic pathogens in an in vitro apical lesion model.
- To determine the direct effects of laser irradiation and associated thermal changes on microbial viability.
Main Methods:
- An in vitro apical lesion model was established using Enterococcus faecalis in semisolid agar.
- The model was inoculated into the apical lesion area.
- The impact of diode laser irradiation and photosensitizer on microbial viability was analyzed, alongside temperature changes.
Main Results:
- A significant reduction in Enterococcus faecalis viability was observed when using the combination of a photosensitizer and diode laser irradiation.
- Laser irradiation led to a temperature increase within the lesion model.
- The observed temperature increases did not exhibit cytotoxic effects on the microorganisms.
Conclusions:
- Diode laser irradiation in conjunction with a photosensitizer demonstrates significant antimicrobial effects against endodontic pathogens in periapical lesions.
- This combination therapy presents a potentially effective method for the clinical management of periapical lesions.
- Photodynamic therapy offers a promising adjunctive treatment modality in endodontics.
