Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026

Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025

Same author

A novel glucose beta-hydroxybutyrate combination improves hypoglycaemia recovery and patient-reported outcomes in type 1 diabetes.

Diabetes, obesity & metabolism·2025

Same author

Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13 TeV with the ATLAS Detector.

Physical review letters·2025

Same author

Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02 TeV with the ATLAS Detector.

Physical review letters·2025

Same author

Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13 TeV with the ATLAS Detector.

Physical review letters·2025

Search research articles

Related Experiment Video

Updated: May 28, 2026

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

High-resolution helical cone-beam micro-CT with theoretically-exact reconstruction from experimental data.

T Varslot¹, A Kingston, G Myers

¹Department of Applied Mathematics, The Australian National University, Canberra, ACT 0200, Australia. trond.varslot@anu.edu.au

Medical Physics

|October 14, 2011

Summary

This study introduces autofocus-corrected, theoretically-exact helical computed tomography (CT) for high-resolution micro-CT imaging. This method overcomes instabilities, enabling faster and more efficient imaging of long objects.

More Related Videos

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Related Experiment Videos

Last Updated: May 28, 2026

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Area of Science:

Medical Imaging
Computational Imaging
X-ray Imaging

Background:

High-resolution tomographic reconstruction in micro-CT has been limited by instabilities.
Previous methods were restricted to low cone-angles, hindering efficiency.

Purpose of the Study:

To demonstrate the viability of optimization-based autofocus for theoretically-exact helical micro-CT reconstruction.
To overcome instabilities that impede high-resolution, theoretically-exact tomographic reconstruction.

Main Methods:

Utilized the theoretically-exact Katsevich 1PI inversion formula for arbitrary cone-angles.
Developed a modified autofocus method to correct for helical trajectory deviations.
Applied software correction for hardware misalignment in virtual projections.

Main Results:

Achieved theoretically-exact reconstruction from experimental data using the Katsevich 1PI (K1PI) inversion formula.
Demonstrated autofocus-corrected helical CT reduces image acquisition time by an order of magnitude.
Enabled high-resolution micro-CT imaging at high cone-angles (∼50°).

Conclusions:

Autofocus-corrected, theoretically-exact cone-beam reconstruction is a practical solution for high-resolution micro-CT.
This approach significantly reduces image acquisition time.
Opens possibilities for efficient imaging of extended objects.