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Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
Published on: October 18, 2012
A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens
Jason Rice1, Myron Christodoulides
1Division of Cancer Sciences, Genetic Vaccines Group, University of Southampton Medical School, Southampton, UK. J.Rice@soton.ac.uk
Abstract:
DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.
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