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Updated: May 28, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Preparative-scale isoelectric trapping by recursive electrophoresis in a compartmentalized system having orthogonal
1Department of Chemistry, Texas A&M University, College Station, TX, USA.
Abstract:
The performance of the current preparative-scale isoelectric trapping systems is limited by the serial arrangement of the separation compartments. A new system has been developed that achieves trapping by recursive electrophoresis in a compartmentalized system (T-RECS). T-RECS features (i) parallel-connected elementary separation heads with independent electrode compartments, feed compartments, and harvest compartments, (ii) orthogonal primary pH gradients and secondary pH gradients, (iii) directionally controlled convective analyte transport along the primary (resolving) pH gradients, and (iv) electrophoretic analyte transport along the secondary (harvesting) pH gradients. The operation of T-RECS has been quantitatively characterized via separation of small molecule ampholytes.
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