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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 28, 2026

Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles

Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles

Published on: March 9, 2019

DNA bimodified gold nanoparticles.

Tao Zhang¹, Yuanchen Dong, Yawei Sun

¹Department of Chemistry, Renmin University of China, Beijing 100872, PR China.

Langmuir : the ACS Journal of Surfaces and Colloids

|October 15, 2011

Summary

This summary is machine-generated.

Researchers developed a new method to link gold nanoparticles (GNPs) using two DNA strands. This DNA-templated assembly allows for precise control over nanoparticle arrangement, creating custom nanostructures.

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Area of Science:

Nanotechnology
Materials Science
Bioconjugation Chemistry

Background:

Gold nanoparticles (GNPs) are widely used in diagnostics and therapeutics.
Controlling the assembly of nanoparticles is crucial for advanced applications.
DNA nanotechnology offers precise control over nanoscale structures.

Purpose of the Study:

To develop a general method for bimodifying gold nanoparticles with two distinct DNA strands.
To enable the creation of ordered nanoparticle assemblies through DNA templating.
To expand the toolkit for DNA-gold nanoparticle conjugates.

Main Methods:

Utilizing a DNA template reaction to attach two different DNA strands to GNPs.
Employing thioctic acid modified DNA strands for attachment via thiol-gold bonds.
Characterizing nanoparticle assemblies using gel electrophoresis, transmission electron microscopy (TEM), and UV-vis spectroscopy.

Main Results:

Successfully achieved bimodification of GNPs with two distinct DNA strands.
Demonstrated the ability to form ordered assemblies, including 5 nm GNP chains, 10 nm GNP chains, and alternating arrangements.
Confirmed the structure and assembly of nanoparticles through various characterization techniques.

Conclusions:

The developed method provides a versatile approach for creating custom DNA-gold nanoparticle conjugates.
This technique allows for precise control over nanoparticle arrangement and assembly.
The findings enrich the field of DNA-nanoparticle conjugates and offer diverse selectivity for future applications.