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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Quantitative Analysis01:12

Quantitative Analysis

Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the method...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Factors that affect quantification of diode array data in comprehensive two-dimensional liquid chromatography using

Hope P Bailey1, Sarah C Rutan, Peter W Carr

  • 1Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA.

Journal of Chromatography. A
|October 15, 2011
PubMed
Summary

Comprehensive two-dimensional liquid chromatography (LC×LC) quantification is improved using chemometric techniques. This approach enhances precision by 2.5-fold and reveals previously undetected peaks in complex samples.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Chemometrics

Background:

  • Comprehensive two-dimensional liquid chromatography (LC×LC) is a rapidly advancing separation technique.
  • Quantification remains a significant analytical challenge in LC×LC, hindering its broader application.
  • Key issues affecting LC×LC quantification include data complexity, retention time shifts, dynamic range limitations, peak overlap, and background signal removal.

Purpose of the Study:

  • To investigate the critical factors impacting peak quantification in LC×LC.
  • To evaluate the effectiveness of chemometric techniques in addressing these quantification challenges.
  • To determine the overall impact of these factors on the quantitative analysis of complex samples using LC×LC.

Main Methods:

  • Analysis of fourteen replicate urine samples to simulate complex matrices.
  • Focus on factors affecting peak quantification: data complexity, retention time shifting, dynamic range, peak overlap, and background signal.
  • Application of chemometric techniques to LC×LC data.

Main Results:

  • Chemometric methods significantly minimized deleterious effects from overlapped peaks, retention time shifts, and background interference.
  • A 2.5-fold increase in quantification precision was achieved with chemometrically resolved data compared to raw data.
  • Sixteen previously non-evident peaks were quantified after chemometric analysis.

Conclusions:

  • Chemometric techniques substantially improve the precision and accuracy of LC×LC quantification.
  • LC×LC, when enhanced by chemometrics, is effective for quantitative analysis of complex samples.
  • Addressing quantification challenges is crucial for the further development and adoption of LC×LC.