Related Experiment Video

Updated: May 28, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Label-free solution-based kinetic study of aptamer-small molecule interactions by kinetic capillary electrophoresis

Jiayin Bao, Svetlana M Krylova, Oren Reinstein

    Analytical Chemistry
    |October 15, 2011
    PubMed

    More Related Videos

    Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry06:39

    Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

    Published on: January 26, 2024

    Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms15:27

    Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

    Published on: April 17, 2017

    Related Concept Videos

    The Equilibrium Binding Constant and Binding Strength02:18

    The Equilibrium Binding Constant and Binding Strength

    The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
    Introduction to Enzyme Kinetics01:19

    Introduction to Enzyme Kinetics

    Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
    The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
    Capillary Electrophoresis: Applications01:30

    Capillary Electrophoresis: Applications

    Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
    Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
    Protein-Drug Binding: Determination Methods01:22

    Protein-Drug Binding: Determination Methods

    Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
    Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...