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Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Published on: March 27, 2013

A quick, simple and unbiased method to quantify C2C12 myogenic differentiation.

Pedro Veliça¹, Chris M Bunce

¹School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. velica@gmail.com

|October 15, 2011

Summary

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Quantify C2C12 cell differentiation using a new image analysis method. This technique measures myotube density via dark pixel counts, offering a faster, unbiased alternative to traditional fusion index analysis.

Area of Science:

Cell Biology
Biotechnology

Background:

C2C12 myoblasts differentiate into myotubes in vitro, a process quantified by the fusion index.
Traditional fusion index analysis is labor-intensive and prone to operator bias.

Purpose of the Study:

Develop a simple, objective method to quantify C2C12 myogenesis.
Establish an alternative to the fusion index for measuring myotube formation.

Main Methods:

Utilized Jenner-Giemsa stained C2C12 cell micrographs.
Employed ImageJ software to generate image histograms.
Quantified myotube density by summing the darkest tones in micrographs.

Main Results:

Myotube density measurements correlated with fusion index during C2C12 differentiation.

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Molecular Imaging to Target Transplanted Muscle Progenitor Cells

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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

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The method accurately reflected changes after treatment with prostaglandin D2, a myogenesis inhibitor.

Conclusions:

Proposed an inexpensive, rapid, and unbiased method for quantifying C2C12 differentiation.
This image analysis technique complements traditional fusion index analysis.