Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2-kW average power capability of a liquid face-cooled, rotating multi-disk amplifier technology.

Optics letters·2026
Same author

Impact of oblique reflections on the generation of isolated attosecond pulses by polarization gating.

Optics letters·2025
Same author

Retraction Note: Burnout components, perceived stress and hair cortisol in healthcare professionals during the second wave of COVID 19 pandemic.

Scientific reports·2025
Same author

Burnout components, perceived stress and hair cortisol in healthcare professionals during the second wave of COVID 19 pandemic.

Scientific reports·2024
Same author

Phase-matching-free ultrashort laser pulse characterization from a transient plasma lens.

Optics letters·2024
Same author

D<sub>3</sub><sup>+</sup> formation through photoionization of the molecular D<sub>2</sub>-D<sub>2</sub> dimer.

Nature chemistry·2023

Related Experiment Video

Updated: May 28, 2026

Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations
13:56

Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations

Published on: October 12, 2019

Conical intersection dynamics in NO2 probed by homodyne high-harmonic spectroscopy.

H J Wörner1, J B Bertrand, B Fabre

  • 1Joint Laboratory for Attosecond Science, National Research Council of Canada and University of Ottawa, Ottawa, Ontario, Canada. woerner@phys.chem.ethz.ch

Science (New York, N.Y.)
|October 15, 2011
PubMed
Summary

High-harmonic spectroscopy reveals electronic dynamics at conical intersections in nitrogen dioxide. This technique tracks molecular changes during photoexcitation and dissociation, offering insights into nonadiabatic chemical reactions.

More Related Videos

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
05:45

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

Related Experiment Videos

Last Updated: May 28, 2026

Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations
13:56

Probe Type II Band Alignment in One-Dimensional Van Der Waals Heterostructures Using First-Principles Calculations

Published on: October 12, 2019

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
05:45

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

Area of Science:

  • Molecular dynamics
  • Ultrafast spectroscopy
  • Quantum chemistry

Background:

  • Conical intersections are fundamental to polyatomic molecule chemistry, influencing reactions and DNA photostability.
  • Real-time studies of electronic dynamics at conical intersections present significant measurement challenges.

Purpose of the Study:

  • To investigate the electronic dynamics of nitrogen dioxide during conical intersection passage.
  • To demonstrate the utility of high-harmonic spectroscopy for probing nonadiabatic chemical pathways.

Main Methods:

  • Utilized ultrafast high-harmonic spectroscopy.
  • Studied photoexcited nitrogen dioxide wave packet dynamics.

Main Results:

  • Observed oscillations in electronic character as the wave packet crossed a conical intersection.
  • Detected the onset of statistical dissociation dynamics at longer time delays.

Conclusions:

  • High-harmonic spectroscopy is a powerful tool for elucidating electronic dynamics along nonadiabatic reaction pathways.
  • The findings provide new methods for studying complex molecular reactions in real-time.