Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 28, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Comparative performance of human papillomavirus DNA testing using novel sample collection methods.

Julia C Gage1, Edward E Partridge, Alfio Rausa

  • 1Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd., MSC 7231, Rockville, MD 20852, USA. gagej@mail.nih.gov

Journal of Clinical Microbiology
|October 15, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cervical cancer burden and elimination readiness in the US-affiliated Pacific Islands: A structured narrative review.

Cancer epidemiology·2026
Same author

Long-term impact of cervical ablation in visual assessment of the squamocolumnar junction: implications for subsequent screening and follow-up.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2025
Same author

Long-term outcomes after cervical cancer screening in El Salvador: primary human papillomavirus screen-and-treat compared with cytology.

BMJ global health·2025
Same author

Determinants of cervical high-risk human papillomavirus positivity among Rwandan women living with human immunodeficiency virus.

PloS one·2025
Same author

Comparing a "See-and-Treat" HPV-Based to VIA-Based Cervical Cancer Prevention Strategies Among Women Living with HIV in Cameroon: A Pilot Study.

Research square·2025
Same author

Supporting our survivors: an evaluation of the facilitators and barriers to advocacy in cervical and breast cancer survivors.

The oncologist·2025

Human papillomavirus (HPV) DNA testing with self-collected samples shows promise for cervical cancer screening in underserved areas. Hybrid Capture 2 (HC2) assay demonstrated higher specificity and better agreement across sample types compared to other HPV tests.

Area of Science:

  • Gynecology
  • Oncology
  • Infectious Diseases

Background:

  • Cervical cancer screening is crucial, especially in underserved populations.
  • Human papillomavirus (HPV) DNA testing is a key screening method.
  • Evaluating alternative sample collection and assay performance is vital for improving accessibility.

Purpose of the Study:

  • To compare HPV DNA assay performance with different sample collection methods for cervical cancer screening.
  • To assess the feasibility of self-collection for HPV testing in the Mississippi Delta region.
  • To identify optimal strategies for primary cervical screening in resource-limited settings.

Main Methods:

  • Three specimen types were collected: clinician-collected cervical, clinician-collected cervicovaginal, and at-home self-collected cervicovaginal.

More Related Videos

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

Related Experiment Videos

Last Updated: May 28, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

  • Specimens were tested using three HPV DNA assays: Hybrid Capture 2 (HC2), Linear Array (LA), and Amplicor.
  • Performance was evaluated by comparing positivity rates and agreement across assays and collection methods.

    • Main Results:

    • No significant differences in HPV positivity were found between clinician-collected and self-collected cervicovaginal specimens across all assays.
    • The HC2 assay showed significantly lower positivity rates compared to LA and Amplicor for both cervicovaginal specimen types.
    • HC2 demonstrated the best agreement between different specimen types, suggesting higher clinical specificity.

    Conclusions:

    • Self-collected cervicovaginal specimens are a viable option for HPV-based cervical cancer screening.
    • The HC2 assay may offer improved clinical specificity for HPV testing, potentially reducing unnecessary referrals.
    • Optimizing HPV testing strategies, including sample collection and assay choice, can enhance screening in underserved populations.