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Updated: May 28, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Clinical application of urinary proteomics/peptidomics
Amaya Albalat1, Harald Mischak, William Mullen
1BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, UK.
Expert Review of Proteomics
|October 18, 2011
Summary
Advancements in proteome analysis technology are driving the discovery of urinary biomarkers for clinical applications. This review highlights technical aspects, study designs, and examples of validated biomarkers from proteomic research.
Area of Science:
- Biomarker Discovery
- Proteomics
- Clinical Chemistry
Background:
- Proteome analysis technologies have advanced significantly, increasing studies on proteome/peptidome analysis for clinically relevant biomarkers.
- Urine is emerging as a key biofluid for clinical proteomics, with initial clinical trials and large-scale patient studies published.
- The transition from potential biomarker discovery to validated, clinically important biomarkers is a critical focus.
Purpose of the Study:
- To summarize technical aspects of biomarker discovery in proteomics.
- To outline the process from potential biomarker identification to clinical validation.
- To discuss experimental design and statistical considerations for clinical endpoints.
Main Methods:
- Focus on capillary electrophoresis coupled to mass spectrometry (CE-MS) for urinary biomarker discovery.
- Review of proteomic and peptidomic analysis techniques.
- Statistical approaches for experimental design in biomarker studies.
Main Results:
- Several examples of proteomic studies identifying urinary biomarkers for clinical applications are presented.
- Emphasis on the utility of CE-MS in discovering and validating urinary biomarkers.
- Discussion of validated urinary biomarkers and their clinical relevance.
Conclusions:
- Technological progress in proteomics facilitates the discovery of clinically relevant urinary biomarkers.
- Rigorous experimental design and statistical validation are essential for translating biomarkers into clinical practice.
- Future studies should address current challenges to advance the clinical utility of urinary proteomics.
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