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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Quantitative proteomics of complex mixtures.
1Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, R3E 0J9, Canada. kcoombs@cc.umanitoba.ca
Expert Review of Proteomics
|October 18, 2011
Summary
Quantitative proteomics enables the study of thousands of proteins in complex biological samples. This review covers methods for reducing sample complexity and common quantitative protein measurement techniques for biological research.
Area of Science:
- Proteomics
- Biotechnology
- Bioinformatics
Background:
- Measuring effector protein molecules is crucial for biological research.
- High-resolution mass spectrometry and bioinformatics advance simultaneous quantitative proteomic analysis.
- Analyzing hundreds to thousands of proteins in complex mixtures elucidates cellular system alterations.
Purpose of the Study:
- To outline strategies for reducing sample complexity in proteomics.
- To describe common quantitative protein measurement methods.
- To provide recent examples of proteomic strategies.
Main Methods:
- Multidimensional separations (gel-based, chromatography-based, multidimensional protein identification technology).
- Quantitative protein measurement techniques including 2D difference in-gel electrophoresis, isotope-coded affinity tags, isotope-coded protein labeling, tandem mass tags, isobaric tags for relative and absolute quantitation, stable isotope labeling of amino acids in cell culture, and label-free methods.
Main Results:
- Quantitative proteomic analysis of complex mixtures is feasible.
- Various methods exist for sample complexity reduction and protein quantitation.
- Recent examples illustrate the application of these proteomic strategies.
Conclusions:
- Quantitative proteomics provides insights into molecular alterations in cellular systems.
- Advances in technology enable comprehensive protein analysis.
- This review serves as a guide to current proteomic methodologies.
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