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Microcantilevers modified by specific peptide for selective detection of trimethylamine
Xin Huang1, Mingfu Li, Xiaohe Xu
1Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, People's Republic of China.
None:
We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.
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