Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microfluidic co-culture system for synaptically segregated neural networks to explore astrocyte-driven neural pathology.

Microsystems & nanoengineering·2026

Same author

Miniaturised electrophoretic analyser with integrated filter-free microfluidic sample treatment for monitoring of inorganic anions in water.

Analytica chimica acta·2025

Same author

Closed-loop peristaltic micro-pump for flow-through sample injection in capillary electrophoresis.

Talanta·2025

Same author

Tailoring molecularly imprinted polymer on titanium-multiwalled carbon nanotube functionalized gold electrode for enhanced chlorophyll determination in microalgae health assessment.

Mikrochimica acta·2024

Same author

Nanoplastic Sample Cleanup by Micro-Electromembrane Extraction across Free Liquid Membranes.

Analytical chemistry·2024

Same author

Methamphetamine detection using portable capillary electrophoresis coupled with a swab-based extraction device.

Talanta·2024

Search research articles

Related Experiment Video

Updated: May 28, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Capillary and microchip electrophoresis: challenging the common conceptions.

Michael C Breadmore¹

¹Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Tasmania, Australia. mcb@utas.edu.au

Journal of Chromatography. A

|October 18, 2011

Summary

Capillary electrophoresis (CE) and microchip electrophoresis (ME) offer advantages over gel electrophoresis (GE) but face challenges compared to liquid chromatography (LC). This review examines their strengths and weaknesses, challenging common perceptions.

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: May 28, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary electrophoresis (CE) is a powerful analytical separation technique.
Microchip electrophoresis (ME) miniaturizes CE, enhancing speed and integration.
CE and ME are alternatives to gel electrophoresis (GE) but often compared unfavorably to liquid chromatography (LC).

Purpose of the Study:

To review the strengths and weaknesses of capillary electrophoresis (CE) and microchip electrophoresis (ME).
To challenge common perceptions regarding the capabilities of CE and ME compared to traditional methods like liquid chromatography (LC).

Main Methods:

Literature review of capillary electrophoresis (CE) and microchip electrophoresis (ME).
Comparative analysis of CE and ME against gel electrophoresis (GE) and liquid chromatography (LC).

Main Results:

CE offers advantages in speed, flexibility, portability, and reduced sample/reagent needs.
ME further improves speed, sample efficiency, and analytical integration.
Commonly held beliefs about CE and ME's inferiority to LC are critically examined.

Conclusions:

CE and ME present viable alternatives to GE with distinct advantages.
Further investigation is needed to fully understand and leverage the potential of CE and ME, potentially altering perceptions of their performance relative to LC.