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Updated: May 28, 2026

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
Published on: January 11, 2018
Surface-enhanced Raman scattering study of riboflavin on borohydride-reduced silver colloids: Dependence of
Fangfang Liu1, Huaimin Gu, Yue Lin
1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Abstract:
The influences of concentration, halide anions and pH on the surface-enhanced Raman scattering (SERS) of riboflavin adsorbed on borohydride-reduced silver colloids were studied. The optimum concentration for the SERS of riboflavin is 10(-6)mol/L while the SERS enhancement varies for different modes. The addition of 0.2mol/L halide (NaCl, NaBr, and NaI) aqueous solutions, leads to a general decrease of the SERS intensity and a change of spectral profile of riboflavin excited at 514.5nm. Riboflavin interacts with the silver surface possibly through the CO and N-H modes of the uracil ring. The SERS spectra of riboflavin were recorded in the 3.4-11.6 pH range. By analyzing several SERS marker bands, the protonated, deprotonated or the coexistence of both molecular species adsorbed on the colloidal silver particles was proved.
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