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Updated: May 28, 2026
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
Potential of fluorescent metalloproteinase substrates for cancer detection
Roopali Roy1, David Zurakowski, Susan Pories
1Program in Vascular Biology and Department of Surgery, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
Objectives:
MMP-2, MMP-9, their complexes and ADAM12 are detected in the urine of breast cancer patients and predict disease status. We assessed the use of FRET-based substrates in an assay to distinguish breast cancer patients from controls.
Design And Methods:
Substrates with varying specificities for MMP-9 and MMP-2 and several ADAMs were screened. Flsub21 and Flsub13, substrates for ADAM12 and ADAM8 respectively, were studied.
Results:
Flsub21 and Flsub13 cleavage activities were detected in the urine of patients with invasive and metastatic breast cancers at significantly higher frequencies compared to controls. Our model predicted probabilities of 90% when both Flsub21 and Flsub13 were positive, 65% when Flsub21 alone was positive, 55% when Flsub13 alone was positive and 20% when both substrates were negative.
Conclusions:
These data suggest the potential utility of FRET substrates to non-invasively identify invasive and/or metastatic breast cancer.
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