Related Experiment Video
Updated: May 28, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Noncontact photoacoustic imaging achieved by using a low-coherence interferometer as the acoustic detector
Yi Wang1, Chunhui Li, Ruikang K Wang
1Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
Abstract:
We report on a noncontact photoacoustic imaging (PAI) technique in which a low-coherence interferometer [(LCI), optical coherence tomography (OCT) hardware] is utilized as the acoustic detector. A synchronization approach is used to lock the LCI system at its highly sensitive region for photoacoustic detection. The technique is experimentally verified by the imaging of a scattering phantom embedded with hairs and the blood vessels within a mouse ear in vitro. The system's axial and lateral resolutions are evaluated at 60 and 30 μm, respectively. The experimental results indicate that PAI in a noncontact detection mode is possible with high resolution and high bandwidth. The proposed approach lends itself to a natural integration of PAI with OCT, rather than a combination of two separate and independent systems.
Related Concept Videos
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

