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R432 is a key residue for the multiple functions of Ndt80p in Candida albicans

Yun Liang Yang1, Chih Wei Wang, Shiang Ning Leaw

  • 1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.

Cellular and Molecular Life Sciences : CMLS
|October 18, 2011
PubMed

Insights

The transcription factor Ndt80p regulates stress genes in Candida albicans. A specific residue (R432) is crucial for its function, suggesting a new antifungal drug target.

Area of Science:

  • Mycology
  • Molecular Biology
  • Pathogenesis

Background:

  • Candida albicans is a major human fungal pathogen causing systemic infections.
  • Ndt80p is a key transcription factor regulating stress-response genes in C. albicans.

Purpose of the Study:

  • To investigate the role of Ndt80p in regulating target genes via the mid-sporulation element (MSE).
  • To determine the significance of the R432 residue in Ndt80p's DNA-binding and functional activities.

Main Methods:

  • Analysis of Ndt80p's direct regulation of target genes like YHB1 using MSE.
  • Complementation studies using the ndt80(R432A) allele to assess the R432 residue's function.

Main Results:

  • Ndt80p directly regulates genes including YHB1 through the MSE.
  • The R432A mutation impaired Ndt80p's ability to bind MSE and complement null mutations.
  • The R432 residue is essential for Ndt80p's roles in cell separation, drug resistance, nitric oxide inactivation, morphogenesis, and virulence.

Conclusions:

  • The R432 residue in Ndt80p is critical for its DNA binding and diverse functions in Candida albicans.
  • Targeting the Ndt80p-MSE interaction presents a potential strategy for developing novel antifungal therapies.

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