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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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Enrichment techniques employed in phosphoproteomics.

Jan Fíla1, David Honys

  • 1Laboratory of Pollen Biology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, Prague 6, Czech Republic.

Amino Acids
|October 18, 2011
PubMed
Summary
This summary is machine-generated.

Phosphoprotein enrichment is vital for studying cellular processes. This article reviews and compares the latest phosphoprotein and phosphopeptide enrichment techniques, highlighting their strengths and weaknesses for comprehensive phosphoproteome coverage.

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Last Updated: May 28, 2026

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Area of Science:

  • Proteomics
  • Cellular Biology
  • Biochemistry

Background:

  • Protein phosphorylation is a key regulator of cellular processes.
  • Phosphoproteins are often low in abundance and difficult to identify.
  • Enrichment strategies are necessary for phosphoprotein and phosphopeptide analysis.

Purpose of the Study:

  • To discuss and compare the most up-to-date phosphoprotein and phosphopeptide enrichment techniques.
  • To highlight the advantages and disadvantages of various enrichment strategies.
  • To reveal the complementary nature of different methods for comprehensive phosphoproteome coverage.

Main Methods:

  • Review of current phosphoprotein enrichment strategies.
  • Discussion of phosphopeptide enrichment techniques.
  • Comparison of affinity chromatography and immunoprecipitation methods.

Main Results:

  • Various enrichment strategies exist, including chemical modifications, affinity chromatography, and immunoprecipitation.
  • Each method has distinct advantages and disadvantages.
  • Optimized techniques enhance the identification of low-abundance phosphoproteins.

Conclusions:

  • Phosphoprotein and phosphopeptide enrichment are crucial for phosphoproteomics.
  • Comparing and optimizing enrichment methods improves phosphoproteome coverage.
  • The complementary nature of different techniques is essential for comprehensive analysis.