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Related Concept Videos

Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into...
Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
Bone Cells and Tissue01:30

Bone Cells and Tissue

Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the periosteum and...
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...

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Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
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Published on: July 14, 2023

Human fetal bone cells in delivery systems for bone engineering.

Diene M H Tenorio1, Corinne Scaletta, Sandra Jaccoud

  • 1Department of Musculoskeletal Medicine, University Hospital of Lausanne, Lausanne, Switzerland.

Journal of Tissue Engineering and Regenerative Medicine
|October 18, 2011
PubMed
Summary

Human fetal bone cells cultured in hyaluronic acid gel and collagen foam showed high biocompatibility and differentiation. Both matrices support cell delivery for bone engineering, with collagen foam ideal for cavities and hydrogels for injections.

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Published on: August 8, 2022

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Developing effective delivery systems for bone regeneration is crucial.
  • Biocompatible scaffolds are needed to support cell survival and function in vivo.
  • Hyaluronic acid and collagen are promising biomaterials for bone tissue engineering.

Purpose of the Study:

  • To compare the biocompatibility of hyaluronic acid gel and collagen foam as matrices for human fetal bone cell culture.
  • To evaluate these matrices as potential delivery systems for bone engineering, particularly for oral applications.
  • To assess cell proliferation, differentiation, and matrix production within the scaffolds.

Main Methods:

  • Human fetal bone cells were cultured in hyaluronic acid (Mesolis®) and collagen foam (TissueFleece®) for up to 4 weeks.
  • Cell survival and differentiation were assessed using proliferation assays and histological staining (Giemsa, von Kossa, ALP).
  • Three-dimensional cell proliferation capacity was compared to monolayer cultures.

Main Results:

  • Human fetal bone cells proliferated in both matrices at approximately 70% of monolayer capacity.
  • Cells exhibited positive ALP and von Kossa staining, indicating differentiation and matrix production.
  • Collagen foam offered better structural support for cavity filling, while hydrogels allowed for injectable delivery.

Conclusions:

  • Both hyaluronic acid gel and collagen foam demonstrate high biocompatibility for human fetal bone cells.
  • These matrices facilitate cell delivery and support cellular differentiation and matrix deposition for bone stimulation.
  • The choice of matrix depends on the specific application, with collagen foam suitable for filling defects and hydrogels for injectable delivery.