Related Experiment Video
Updated: May 28, 2026

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
Published on: April 27, 2021
Generalized methods and solvers for noise removal from piecewise constant signals. I. Background theory
1Department of Physics and Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, UK.
None:
Removing noise from piecewise constant (PWC) signals is a challenging signal processing problem arising in many practical contexts. For example, in exploration geosciences, noisy drill hole records need to be separated into stratigraphic zones, and in biophysics, jumps between molecular dwell states have to be extracted from noisy fluorescence microscopy signals. Many PWC denoising methods exist, including total variation regularization, mean shift clustering, stepwise jump placement, running medians, convex clustering shrinkage and bilateral filtering; conventional linear signal processing methods are fundamentally unsuited. This paper (part I, the first of two) shows that most of these methods are associated with a special case of a generalized functional, minimized to achieve PWC denoising. The minimizer can be obtained by diverse solver algorithms, including stepwise jump placement, convex programming, finite differences, iterated running medians, least angle regression, regularization path following and coordinate descent. In the second paper, part II, we introduce novel PWC denoising methods, and comparisons between these methods performed on synthetic and real signals, showing that the new understanding of the problem gained in part I leads to new methods that have a useful role to play.
Related Concept Videos
Reconstruction of Signal using Interpolation
Deconvolution
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Classification of Signals
A continuous-time signal holds a value at every instant in time, representing information seamlessly. In contrast, a discrete-time signal holds values only at specific moments, often denoted as x(n), where...
Even and Odd Signals
Linear time-invariant Systems
The input-output behavior of an LTI system can be fully defined by its response to an impulsive excitation at its input. Once this impulse response is known, the system's reaction to any other input can be calculated...
Sampling Continuous Time Signal
In the...

