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Updated: May 28, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
TACN-containing cationic lipids with ester bond: preparation and application in gene delivery
Qin-Fang Zhang1, Wei-Han Yang, Wen-Jing Yi
1Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
Abstract:
A series of novel cationic lipids based on 1,4,7-triazacyclononane (TACN) with different hydrophobic chains were synthesized via the formation of a biodegradable ester bond. These lipids were found to have good buffering capacity at the pH range of 5.0-6.5, which is similar to that of the acidic endosomal compartments. The liposomes formed from these lipids and DOPE could condense DNA into nanoparticles with proper sizes. In vitro experiments showed moderate to good gene transfection efficiency of the formed lipoplexes. The structure-activity relationships of this type of lipids were discussed.
