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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and
Harita Rao1, Anupam A Sawant, Arun A Tanpure
1Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, Pune 411021, India.
Abstract:
Direct incorporation of azide groups into RNA oligonucleotides by in vitro transcription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are described. This postsynthetic labeling protocol is robust and modular, and offers an alternative access to RNA labeled with biophysical probes.
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