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Updated: May 28, 2026

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Efficient protein transduction method using cationic peptides and lipids.

Kei Yamaguchi¹, Mari Inoue, Naoki Goshima

¹Department of Research and Development, Japan Biological Informatics Consortium (JBiC), 2-4-32 Aomi, Koto-Ku, Tokyo 135-8073, Japan.

Journal of Biomedicine & Biotechnology

|October 19, 2011

Summary

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We developed a novel peptide and lipid system for efficient protein delivery into cells. This method overcomes endosomal entrapment, enhancing intracellular protein delivery and organelle targeting.

Area of Science:

Biochemistry
Cell Biology
Biotechnology

Background:

Protein transduction domains (PTDs) facilitate cell membrane penetration but often lead to endosomal entrapment.
Efficient intracellular delivery of proteins to organelles remains a significant challenge in cell biology.

Purpose of the Study:

To develop a novel protein delivery system that enhances the proton sponge effect for endosomal escape.
To improve the intracellular delivery efficiency of cargo proteins beyond endosomal vesicles.

Main Methods:

Utilized a mixture of Wr-T transporter peptide and a cationic lipid reagent.
Investigated the enhancement of the proton sponge effect for endosomal rupture.
Assessed the delivery efficiency of various cargo proteins into living cells.

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Last Updated: May 28, 2026

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

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Main Results:

The peptide-lipid mixture demonstrated efficient delivery of diverse cargo proteins into cells.
The system effectively promoted endosomal escape, releasing proteins from endosomes.
Enhanced intracellular protein delivery was achieved, overcoming previous limitations.

Conclusions:

The novel Wr-T peptide and cationic lipid system represents an effective strategy for intracellular protein delivery.
This approach overcomes endosomal entrapment, enabling broader applications in cell-based research and therapeutics.
The enhanced proton sponge effect is crucial for successful endosomal release and protein delivery.