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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
Lipase Activity among Bacteria Isolated from Amazonian Soils
André Luis Willerding1, Luiz Antonio de Oliveira, Francisco Wesen Moreira
1Biochemistry and Molecular Biology Coordination, Amazon Biotechnology Center (CBA), Avnue Gov. Danilo Areosa, 690 Distrito Industrial, 69075351 Manaus, AM, Brazil.
Abstract:
The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41%) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation.
Insights
Researchers screened 440 Amazonian bacteria, identifying 75 lipase-positive strains. The best lipase activity occurred at 30°C within 72 hours, highlighting microbial potential for biotransformation.
Area of Science:
- Microbiology
- Biotechnology
- Enzymology
Background:
- The Amazon region harbors diverse microbial communities.
- Lipases are crucial enzymes with broad industrial applications.
- Screening for novel lipase-producing microorganisms is essential for biotechnological advancements.
Purpose of the Study:
- To isolate and characterize lipase-producing bacteria from the Amazon region.
- To evaluate the enzymatic activity and optimal conditions for selected bacterial strains.
- To identify potential microbial resources for industrial applications, such as oil biotransformation.
Main Methods:
- Isolation and screening of bacteria from various Amazonian locations.
- Qualitative lipase assays on Petri dishes.
- Quantitative assays using p-nitrophenyl palmitate (p-NPP).
- Enzymatic index determination at different temperatures (30-45°C) over 15 days.
Main Results:
- Out of 440 strains, 181 were selected for lipase assay, with 75 (41%) showing positive results.
- Highest lipase activity was observed within 72 hours at 30°C.
- Twelve strains exhibited an enzymatic index equal to or greater than the reference standard.
- Selected strains were further analyzed quantitatively and showed potential for use with Amazonian oleaginous substrates.
Conclusions:
- The Amazon region is a promising source of potent lipase-producing bacteria.
- Optimized conditions (30°C, 72 hours) enhance lipase activity.
- Selected bacterial strains demonstrate significant potential for biotechnological applications, including oil biotransformation.
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