Related Experiment Video
Updated: May 28, 2026
Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
Published on: February 14, 2011
[Preparation and identification of monoclonal antibody against hMOF]
Hou-Liang Sun1, Da-Peng Wei, Xiao-Hong Zhang
1Department of Immuniology, Chongqing Three Gorges Medicine Colloge, Wanzhou 404016, China.
Objective:
To prepare and identify the monoclonal antibody (mAb) against hMOF.
Methods:
BALB/C mice were immunized with protein from the spleen cells isolated and fused with SP2/0 cells. After several rounds of screening and cloning, the hybridoma cell strain secreting anti-hMOF mAb was obtained. Its specificity was evaluated with ELISA and Western blot, and the titer, immunoglobulin subtype and affinity of the mAb were measured.
Results:
One cell strains of hybridoma were obtained and named as 4C1C8. The anti-hMOF mAb secreted by the hybridoma cell strain was identified as IgG1 subtype. The mAb titers in ascitic fluid were 1 409600, as determined with ELISA with an affinity reaching to 7.65 x 10(6) L/mol. Western blot demonstrated that the antibodies could specifically recognize the immunogen. The cell immunohistochemistry proved that the antibody could recognize the hMOF antigens expressed on the normal cells HL7702.
Conclusion:
The success in anti-hMOF mAb preparation provides the basis for further study of hMOF.
