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[Screening of plant pathogenic fungi by ginsenoside compound K production]
Yuanchao Yang1, Yingping Wang, Meixia Yan
1Institute of Special Wild Economic Animals and Plants CAAS, Jilin 132109, China. yychao_2004@126.com
Objective:
To screen a new strain which can transform panaxadiol saponins into the rare ginsenoside compound K.
Method:
The total saponins in stems and leaves of Panax notoginseng was used as a substrate in the liquid state fermentation process, and the results were detected by TLC and HPLC-ELSD to screen a strain from twelve plant pathogenic fungi which can produce ginsenoside compound K.
Result:
Fusarium moniliforme was found to transform the total saponins to ginsenoside compound K efficiently in the all twelve fungal strains. In the fermentation process, ginsenoside Rb1 was transformed almost completely, and the content of ginsenoside Rd was decreasing evidently.
Conclusion:
F. moniliforme is selected as a new high-yield strain. It is expected to be used to produce the high activity infrequent ginsenoside compound K and to improve the content of active principles in medicinal plants.
