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Updated: May 28, 2026
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Analysis of flavonoids in platycladi cacumen by UPLC-MS]
Mingqiu Shan1, Wen Qian, Jing Gao
1Nanjing University of Chinese Medicine, Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing 210046, China. shanmingqiu@163.com
Objective:
To investigate the flavonoids in Platycladi Cacumen.
Method:
The constituents in Platycladi Cacumen were determined by UPLC-MS. A Waters BEH C18 column (2.1 mm x 150 mm, 1.7 microm) was used with a gradient elution of methanol-water containing 0.2% formic acid. The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.
Result:
Eleven constituents were identified.
Conclusion:
In this study, the main flavonoids in Platycladi Cacumen were separated by UPLC, and identified through the information of mass number and UV spectra. With the information of MS/MS from flavonoids, the modes of breaking up flavonoids were discussed. It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.
