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Updated: May 28, 2026
Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
[Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC]
Haijian Yuan1, Yigang Chen, Baochang Cai
1Taizhou Polytechnic College, Taizhou 225300, China. yuanjian8101@163.com
Objective:
A new method for simultaneous determination of solasonine (1), solamargine (2) and khasianine (3) in Solanum Nigrum by reversed-phase HPLC was developed.
Method:
The samples were separated at 30 degrees C on Agilent Zorbax SB C18 (4.6 mm x 150 mm, 5 microm) column with acetonitrile-water-phosphoric as mobile phase. Flow rate was 1.0 mL x min(-1) and the detection wavelength was 205 nm.
Result:
There was good linearity between the peak area and concentration at the ranges of 0.860-10.320 microg (r = 0.999 7), 0.726-8.710 microg (r = 0.999 7), 0.856-10.270 microg (r = 0.999 7) for 1, 2 and 3 respectively. The average recoveries of 1, 2 and 3 were 101.04%, 99.65%, 100.17%.
Conclusion:
The method is rapid, simple and accurate, and it can be used for the evaluation of Solanum Nigrum L.
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