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Updated: May 28, 2026
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
[Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS]
Minxia Zheng1, Zhe Chen, Pei Liu
1National Clinical Research Base of Traditional Chinese Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
Objective:
To investigate the monoterpene glycosides in Paeonia lactflora by UPLC-MS/MS.
Method:
An Acquity UPLC BEH C18 column (2.1 mm x 50 mm) with 1.7 microm particle size was used. The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode. The flow rate was 0.4 mL x min and the chromatographic run time was 9 min for one run. The mass spectrometer equipped with an eletrospray ion source in negative ion mode.
Results:
Totally six glycosides were analyzed and identified by the established UPLC-MS/MS method.
Conclusion:
The method was rapid, sensitive, and extremely useful for rapid identification of glycosides in P. lactiflora.
