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Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
Monoclonal antibodies specific for hepatitis B e antigen and hepatitis B core antigen
Ibrahim Sogut1, Ibrahim Hatipoglu, Gungor Kanbak
1Genetic Engineering and Biotechnology Institute, Tubitak Marmara Research Center, Gebze-Kocaeli, Turkey.
Researchers developed two new monoclonal antibodies (12E5 and 16F8) for detecting Hepatitis B virus (HBV) core antigen (HBcAg) and e antigen (HBeAg). These antibodies show potential for improving clinical diagnosis of HBV infection.
Area of Science:
- Immunology
- Virology
- Biotechnology
Background:
- Hepatitis B virus (HBV) infection remains a global health concern, with over 2 billion people infected worldwide.
- Serologic markers like anti-HBc (antibody to HBV core antigen) and anti-HBe (antibody to HBV e antigen) are vital for diagnosing HBV infection, indicating chronicity and active viral replication, respectively.
Purpose of the Study:
- To develop and characterize novel monoclonal antibodies (MAbs) for the detection of Hepatitis B e antigen (HBeAg) and Hepatitis B core antigen (HBcAg).
- To assess the potential of these MAbs for use in the clinical diagnosis of HBV infection.
Main Methods:
- Hybridoma technology was employed to generate MAbs against HBeAg and HBcAg using BALB/c mice immunized with HBeAg.
- Monoclonal antibodies were produced at scale, purified using affinity chromatography, and subjected to epitope analysis.
Main Results:
- Two hybridoma clones, 12E5 and 16F8, secreting antibodies against HBeAg and HBcAg were successfully developed.
- The immunoglobulin types were identified as IgG2a and IgG1, respectively.
- Epitope analysis confirmed the specificity of the MAbs for HBcAg and HBeAg detection.
Conclusions:
- The developed monoclonal antibodies, 12E5 and 16F8, are effective in detecting HBcAg and HBeAg.
- These MAbs hold significant potential for enhancing the clinical diagnostic tools for Hepatitis B virus infection.
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