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Related Experiment Video

Updated: May 28, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

Active immunization against IL-23p19 improves experimental arthritis.

Rojo Anthony Ratsimandresy¹, Emilie Duvallet, Eric Assier

¹Équipe Génomique, Bioinformatique et Applications, Chaire de Bioinformatique, Conservatoire National des Arts et Métiers 292, rue Saint-Martin, 75003 Paris, France. rratsima@gmail.com

|October 20, 2011

Summary

Targeting the IL-23p19 subunit with a peptide-based vaccine (IL23-K1) significantly reduced arthritis severity in a collagen-induced arthritis (CIA) mouse model. This approach shows promise for treating autoimmune diseases like rheumatoid arthritis.

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Area of Science:

Immunology
Autoimmunity
Vaccine Development

Background:

Interleukin-23 (IL-23) is a pro-inflammatory cytokine crucial for Th17 cell differentiation, implicated in autoimmune diseases.
IL-23 shares the IL-12/23p40 subunit with IL-12 and has a unique subunit, IL-23p19.
Previous studies showed therapeutic benefits from targeting TNF-α and IL-1β in animal models of rheumatoid arthritis.

Purpose of the Study:

To evaluate the efficacy of peptide-based vaccines targeting the IL-23p19 subunit in collagen-induced arthritis (CIA).

Main Methods:

Bioinformatic modeling identified two peptides in the murine IL-23p19 subunit.
Peptides were conjugated to keyhole limpet hemocyanin (KLH) to create vaccines IL23-K1 and IL23-K2.
DBA/1 mice were immunized with vaccines, and CIA was induced using bovine type II collagen (CIIb). Disease progression, antibody levels, cytokine mRNA, and T-cell populations were assessed.

Main Results:

The IL23-K1 vaccine significantly reduced clinical arthritis scores and protected against joint destruction and inflammation.
Mice immunized with IL23-K1 produced higher levels of anti-IL-23 antibodies compared to IL23-K2.
IL23-K1 immunization increased IL-10 mRNA in the spleen without affecting IL-17 levels; spleen T-cell populations remained unchanged.

Conclusions:

Targeting the IL-23p19 subunit via vaccination demonstrates a protective effect in collagen-induced arthritis.
This IL-23p19-specific vaccination strategy presents a potential therapeutic avenue for rheumatoid arthritis.