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Related Experiment Video

Updated: May 28, 2026

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
11:06

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Published on: May 2, 2017

Laparoscopic retroperitoneal/mesenteric lymph node sampling: a safe and effective technique.

R Durai1, N Mir, P C H Ng

  • 1Department of Surgery and Haematology, University Hospital Lewisham, Lewisham High Street, London, SE13 6LH, UK. dr_durai@yahoo.com

Singapore Medical Journal
|October 20, 2011
PubMed
Summary
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Laparoscopic retroperitoneal lymph node biopsy provides adequate tissue for diagnosis when needle biopsies fail. This minimally invasive approach is safe and effective for assessing lymph node conditions.

Area of Science:

  • Surgical Oncology
  • Minimally Invasive Surgery
  • Diagnostic Pathology

Background:

  • Traditional needle aspiration and core biopsies often yield insufficient tissue for accurate retroperitoneal lymph node tumor typing.
  • Assessing retroperitoneal lymph nodes is crucial for diagnosing various conditions.

Purpose of the Study:

  • To demonstrate the feasibility and safety of using a laparoscopic approach for retroperitoneal lymph node biopsy.
  • To obtain an adequate volume of lymph node tissue for definitive diagnosis and typing.

Main Methods:

  • A laparoscopic retroperitoneal lymph node biopsy procedure was performed on 12 patients over five years.
  • Utilized a Veress needle for pneumoperitoneum and multiple trocars under laparoscopic guidance for nodal tissue access.

Related Experiment Videos

Last Updated: May 28, 2026

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
11:06

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Published on: May 2, 2017

Main Results:

  • The procedure was successful in 11 of 12 patients, yielding an average of 1.7 cm³ of tissue per patient.
  • Histology was adequate in all successful cases, aiding rapid diagnosis of conditions including lymphoma, sarcoidosis, and Castleman's disease.
  • One conversion to open procedure occurred due to bleeding in a patient with undiagnosed portal hypertension.

Conclusions:

  • Laparoscopic biopsy is a viable and safe option for obtaining adequate retroperitoneal lymph node tissue.
  • This technique is particularly valuable when needle biopsies are insufficient or impossible.
  • Laparoscopy provides access to perihepatic and perisplenic regions for lymph node assessment.